T3 RNA Polymerase

P2083_T3-RNA-Polymerase--1-000u_3
customize-this-small
View information on global supply logistics

DNA-Dependent RNA Polymerase Specific for T3 Promoter Sequences

  • Exhibits extremely high affinity and specificity for T3 promoter sequences
  • >90% pure as determined by SDS polyacrylamide gel electrophoresis
  • Free of detectable levels of contaminating RNase and DNase activity (<1% release)
  • Supplied at a concentration of 10–20u/μl (Cat.# P2083) and 80u/µl (Cat.# P4024)

Size

Catalog number selected: P2083

$ 62.00
Your price:
Add to Cart
This product is discontinued
Add to Helix
This product is available under our Early Access program - Learn More
This product is available under our Catalog (FT) program - Learn More
T3 RNA Polymerase
1,000u
$ 62.00
Your price: Log in
Change Configuration

T3 RNA Polymerase is a DNA-dependent RNA polymerase that synthesizes RNA using only T3 DNA or DNA templates cloned downstream from a T3 promoter. The polymerase can incorporate 32P, 33P, 3H and 35S nucleoside triphosphates during transcription. T3 RNA Polymerase is provided with 100mM DTT and Transcription Optimized 5X Buffer: 200mM Tris-HCl (pH 7.9 at 25°C), 30mM MgCl2, 10mM spermidine, 50mM NaCl.

Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/

Applications

  • Generation of templates for in vitro translation.
  • Generation of probes for nucleic acid hybridizations.
  • Generation of RNA processing substrates.
  • Generation of antisense RNA.

References

  1. Butler, E.T. and Chamberlin, M.J. (1982) J. Biol. Chem. 257, 5772–8.
  2. Melton, D.A. et al. (1984) Nucl. Acids Res. 12, 7035–56.

Storage Buffer: 20mM potassium phosphate (pH 7.7 at 25°C), 1.0mM EDTA, 10mM DTT, 100mM NaCl, 0.1% (v/v) Triton® X-100 and 50% (v/v) glycerol.

Source: Recombinant E. coli strain.

QC Tests: Activity, SDS-PAGE/purity, DNase, RNase, endonuclease, transcription.

Unit Definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 5nmol of CTP into acid-insoluble product in 60 minutes at 37°C in a total volume of 100μl. The reaction conditions are: 40mM Tris-HCl (pH 7.9 at 25°C), 6mM MgCl2, 10mM DTT, 10mM NaCl, 2mM spermidine, 0.05% Tween® 20, 0.5mM each of ATP, GTP, CTP, and UTP, 0.5μCi [3H]CTP and 2μg supercoiled pSP6/T3 vector DNA.

Specifications

You are viewing: P2083 Change Configuration

What's in the box?

Item Part # Size Concentration Available Separately

DTT

P117B 1 × 250μl 100mM View Product

Transcription Optimized 5X Buffer

P118B 1 × 500μl View Product

T3 RNA Polymerase

P208C 1 × 1,000u

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: P4024 Change Configuration

What's in the box?

Item Part # Size Concentration Available Separately

DTT

P117B 1 × 250μl 100mM View Product

Transcription Optimized 5X Buffer

P118B 1 × 500μl View Product

T3 RNA Polymerase (HC)

P402A 1 × 2,500u

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Let's find the product that meets your needs.

Talk to a Scientist

scientist-uk-manos

Manos

UK