MagneGST™ Protein Purification System

V8604_MagneGST--Protein-Purification-System_3
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MagneGST™ Protein Purification System

One-Step Protein Purification from 1–50ml of Culture

The MagneGST™ Protein Purification System provides a simple, rapid and reliable method for the purification of glutathione-S-transferase (GST) fusion proteins. Immobilized glutathione paramagnetic particles (MagneGST™ Particles) are used to isolate GST-tagged protein directly from a crude or cleared lysate using either a manual or automated procedure and requires use of a magnetic stand. GST-tagged proteins can be purified on a small scale from 1ml of culture or on a large scale using more than 50ml of culture. Samples also can be processed using a robotic platform. MagneGST™ particles are supplied as a 25% slurry and have a binding capacity of 5–10mg of GST protein per 1ml of settled resin.

The benefits of this system include one-step purification of multiple samples with easy handling, no need for lysate clearing and a scalable system that can be used with 1–50ml of cell culture.

For more information, see the Protocols & Applications Guide.

Note: This product requires the use of a magnetic stand that is not included. 

Applications

  • Protein purification of glutathione-S-transferase fusion proteins from cleared or crude E. coli cell lysate.
  • High-throughput purification of GST-tagged proteins using automated platforms.

 

Purification of GST-ubiquitin and GST-luciferase.

Purification of GST-ubiquitin and GST-luciferase.

Specifications

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What's in the box?

Item Part # Size Available Separately

RQ1 RNase-Free DNase

M610A 1 × 1,000u View Product

MagneGST™ Cell Lysis Reagent

V481A 1 × 12ml

MagneGST™ Glutathione Particles

V861A 1 × 4ml View Product

MagneGST™ Binding/Wash Buffer

V870A 1 × 80ml

Glutathione

V871A 1 × 2ml

Tris Buffer, 0.5M (pH 8.1)

V872A 1 × 2ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Protocol for detailed storage recommendations.

Specifications

You are viewing: V8603 Change Configuration

What's in the box?

Item Part # Size Available Separately

RQ1 RNase-Free DNase

M610A 1 × 1,000u View Product

MagneGST™ Cell Lysis Reagent

V481B 1 × 60ml

MagneGST™ Glutathione Particles

V861B 2 × 10ml View Product

MagneGST™ Binding/Wash Buffer

V870B 2 × 200ml

Glutathione

V871B 1 × 10ml

Tris Buffer, 0.5M (pH 8.1)

V872B 1 × 10ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Protocol for detailed storage recommendations.

Resources

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