ΦX174 DNA/HinfI Dephosphorylated Markers are prepared by digesting double-stranded ΦX174 DNA to completion with HinfI. The DNA fragments are then treated with calf intestinal alkaline phosphatase, phenol:chloroform-extracted, ethanol-precipitated and resuspended in TE buffer, making the markers ready for 5´ end-labeling. The 20 DNA fragments range in size from 24–726bp. The markers are not intended for use in quantitative analysis.
This marker is especially convenient for applications such as primer extension, requiring DNA or RNA size estimations.
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ΦX174 DNA/HinfI Dephosphorylated Markers
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10mM Tris-HCl (pH 8.0), 1mM EDTA.
Store at –20°C.
For product intended use please see Patents & Disclaimers tab.
E3511 For Research Use Only. Not for Use in Diagnostic Procedures.