Protease Assays

Investigate Your Protease

The Protease-Glo™ Assay is a sensitive, fast, and easy method to interrogate proteases with or without P' requirements.

• Technical Article
• Product Details + Ordering

Proteasome-Glo™ Assay

Measure the chymotrypsin-like, trypsin-like and caspase-like activities associated with the proteasome complex in cultured cells. Biochemical versions are also available.

• Technical Article
• Product Details + Ordering

DUB-Glo™ Protease Assay

An easy assay for DUB, SENP or NEDP Activity. Measures activity of deubiquitinating (DUB), deSUMOylating (SENP) and deneddylating (NEDP) proteases.

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Luminescent Caspase Assays

The Caspase-Glo^® Assays monitor toxic effects of compounds at high-throughput capacities, or one sample at a time, in as little as 15 minutes.

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• Product Details + Ordering

Calpain-Glo™ Protease Assay

Measure calpain I (μ) and II (m) activities with a single addition of reagent to test samples.

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Custom Assay Services

Let us partner with you to develop a complete biology-driven, Promega-technology-enabled custom solution: Faster time to data; Fewer in-house resources; Flexible approach and offerings.

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In this article we demonstrate use of the Protease-Glo™ Assay to detect protease activity, evalulate substrate specificity and screen for protease small-molecule modulators.

This tool converts a desired amino acid sequence, representing a protease recognition sequence of interest, into two complementary DNA oligonucleotide sequences.