Anti-βIII Tubulin mAb

Anti-βIII Tubulin mAb is a protein G-purified IgG₁ monoclonal antibody (from clone 5G8) raised in mice against a peptide (EAQGPK) corresponding to the C-terminus of βIII tubulin. It is directed against βIII tubulin, a specific marker for neurons. The major use of this antibody is for labeling neurons in tissue sections and cell culture. The antibody has been tested to perform in frozen and paraffin-embedded sections of rat brain, cerebellum and spinal cord, human and rat fetal CNS progenitor cell cultures and adult human paraffin-embedded brain.

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Anti-βIII Tubulin mAb is a protein G-purified IgG₁ monoclonal antibody (from clone 5G8) raised in mice against a peptide (EAQGPK) corresponding to the C-terminus of βIII tubulin. It is directed against βIII tubulin, a specific marker for neurons. The major use of this antibody is for labeling neurons in tissue sections and cell culture. The antibody has been tested to perform in frozen and paraffin-embedded sections of rat brain, cerebellum and spinal cord, human and rat fetal CNS progenitor cell cultures and adult human paraffin-embedded brain.

Features - Benefits

• Immunogen: Peptide corresponding to the C-terminus (EAQGPK) of βIII tubulin.
• Antibody Form: Mouse monoclonal IgG₁ (clone 5G8), 1mg/ml in PBS containing no preservatives.
• Specificity: Cross-reacts with most mammalian species. Does not label nonneuronal cells (e.g., astrocytes).

Applications

(Suggested working dilution)

• Immunocytochemistry (1:2,000).
• Immunohistochemistry (1:2,000).
• Western blotting (1:1,000).

For more information, see the Protocols & Applications Guide.

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