The TnT® Systems are convenient single-tube systems for eukaryotic-based, cell-free protein expression. To use these systems, circular plasmid DNA containing a T7, T3 or SP6 promoter, or a PCR-generated fragment, is added to an aliquot of the appropriate TnT® Quick Master Mix and incubated for 1 hour. Expressed protein are then ready to use.
Quickly characterize multiple protein:protein interactions by expressing the protein of interest as a fusion protein (typically in E. coli) and the potential interacting prey(s) using TnT® Systems.
Avoid transfection and the generation of cell lysates by expressing both the bait and prey proteins in TnT® Systems and then use appropriate antibodies for immunoprecipitation.
Electrophoretic Mobility Shift Assay (Gel Shift)
Complement other techniques used to analyze protein:nucleic acid interactions by expressing protein in TnT® Systems. Incubate with labeled DNA/RNA fragment and observe migration patterns through a non-denaturing polyacrylamide gel.