The Janelia Fluor® HaloTag® Ligands, including the JFX dyes, are engineered to characterize HaloTag® fusion proteins in endogenous settings. The Janelia Fluor® HaloTag® dyes span the visible spectrum, and their distinct fluorogenic attributes and versatility make them ideal for labeling cell-permeable proteins.
The JFX dyes (analogs of the Janelia Fluor® dyes) incorporate deuterium into the alkylamino substituents of rhodamines, inhibiting photochemically induced spectral shifts and reducing irreparable photobleaching. The enhanced photostability of the Janelia Fluor® and JFX dyes makes them invaluable for detection and single-molecule imaging studies in live cells.
These dyes have been used in a variety of imaging methods, including techniques that are important for studies involving fixed, live-cell and in vivo samples:
- Super-resolution and high-resolution imaging
- Standard confocal imaging
- FACS
- In-gel detection with a fluoroimager
A notable feature of the Janelia Fluor® and JFX dyes is their ability to support sequential labeling of proteins with different colored ligands via pulse-chase labeling. This process facilitates real-time monitoring of protein dynamics, such as trafficking and turnover.