FluoroTect™ Green_Lys in vitro Translation Labeling System

The FluoroTect™ Green_Lys in vitro Translation Labeling System allows for the fluorescent labeling and detection of proteins synthesized in vitro. The system is based on a lysine-charged tRNA that is labeled at the ε position of the lysine with the fluorophore BODIPY®-FL. Fluorescent lysine residues will be incorporated into synthesized proteins during in vitro translation reactions, eliminating the need for radioactivity.

Detection of the labeled proteins is accomplished in 2–5 minutes directly "in-gel" by use of a laser-based fluorescent gel scanner. This eliminates any requirements for protein gel manipulatio...

Expand to Read More »

The FluoroTect™ Green_Lys in vitro Translation Labeling System allows for the fluorescent labeling and detection of proteins synthesized in vitro. The system is based on a lysine-charged tRNA that is labeled at the ε position of the lysine with the fluorophore BODIPY®-FL. Fluorescent lysine residues will be incorporated into synthesized proteins during in vitro translation reactions, eliminating the need for radioactivity.

Detection of the labeled proteins is accomplished in 2–5 minutes directly "in-gel" by use of a laser-based fluorescent gel scanner. This eliminates any requirements for protein gel manipulation such as fixing/drying or any safety, regulatory and waste disposal issues associated with the use of radioactively labeled amino acids use. The convenience of "in-gel" detection also avoids the time-consuming electroblotting and detection steps of conventional non-isotopic systems.

Features - Benefits

• Fast: Data can be obtained in minutes, eliminating overnight exposures associated with radioactive-based systems or time-consuming steps utilized by traditional non-isotopic methodologies.
• Convenient: Results based on "in-gel" detection. No requirement to transfer, fix, or dry gels.
• Non-Radioactive: No safety, regulatory or waste disposal issues associated with radioactivity.
• Flexible: The modified charged tRNA can be used with a variety of Promega translation systems including: Rabbit Reticulocyte Lysate, TnT® Coupled Transcription/Translation System, Wheat Germ Extract and E. coli S30 Extract.

Applications

For more information, see the Protocols & Applications Guide.

« Collapse Content