Improvements in molecular analysis techniques have changed the "bottlenecks" in clinical and basic research. Until recently, analysis required the greatest amount of time and labor. Today however, the most time-consuming and laborious step is often DNA purification, especially when high-throughput processing is required. This article describes two new high-throughput, 96-well, walkaway automated systems for genomic DNA purification. The MagneSil® Blood Genomic, Max Yield System is designed for high-yield genomic DNA purification, purifying 4–9µg of DNA from 200µl of human whole blood and other samples. The MagneSil®> ONE, Fixed Yield Blood Genomic System is designed to purify a "fixed DNA yield", 1.0µg ± 50%, from 60µl of whole blood. Both systems are designed to be implemented on a robotic liquid handler such as the Beckman Coulter Biomek® FX.
Promega Notes 85, 7–10.
Susan Koller, Jacqui Sankbeil, Hemanth Shenoi and Rex Bitner