Maxwell® 16 Polyhistidine Protein Purification Kit Technical Manual

Literature # TM285

The Maxwell® 16 Polyhistidine Protein Purification Kit is used with the Maxwell® 16 Instrument to provide an easy method for efficient, automated purification of polyhistidine-tagged protein from bacterial cultures and other sample types, including mammalian cells, insect cells and culture medium. The kit also may be used for the purification of HQ-tagged proteins from bacterial cultures with some reagent modifications. The HQ tag contains three histidine and three glutamine residues (HQHQHQ). The Maxwell® 16 Instrument is supplied with preprogrammed purification procedures and designed for use with predispensed reagent cartridges, maximizing simplicity and convenience. The instrument can process up to 16 samples in approximately 40 minutes, and the purified protein is compatible with common downstream applications, including polyacrylamide gel electrophoresis and Western blot analysis.