Ultra-High-Throughput Profiling of Compounds Using Luminescent Assays Scientific Poster

Abstract

Brad Larson¹, Tracy Worzella¹, and Mark Chong^2
1Promega Corporation, Madison, WI, ²Aurora Discovery, San Diego, CA

Here we demonstrate the use of Promega luminescent, as well as fluorescent, HTS assays for profiling test compounds in a miniaturized ultra-high-throughput setting. Aurora Discovery’s BioRAPTR FRD™ Workstation was used to dispense cells, test compounds and assay reagents in a 1536-well format. IC₅₀ calculations were performed for each compound and assay combination. Results show that IC_50s from assays performed in a miniaturized uHTS setting can be used to create a clearer picture of the properties for individual compounds.