Citations Search

Notes: The authors of this study developed a model for UVB-induced premature senescence of skin human diploid fibroblasts. Markers of senescence were expressed in the system, and the authors were able to detect a common mitochondrial DNA 4,977-bp deletion that is associated with oxidative damage. After a series of ten UVB stresses, total RNA was prepared from the cells using the RNAgents^® Total RNA Isolation System and used for RT-PCR to detect differentially expressed genes. Forty-four stress or senescence-associated genes were identified that were differentially expressed between UVB irradiated and untreated cells including c-fos, c-jun, insulin-like growth factor binding protein 3, several HSPs, genes involved in protection from oxidative stress, and the type II receptor of TGF-β. The Anti-ACTIVE^® Caspase-3 pAb was used to assess whether the UVB treatment or incubation with TGF-β1 led to apoptosis in this system. (3670)

Notes: The DeadEnd™ Fluorometric TUNEL System was used to label apoptotic Hela cells that were infected with C. trachomatis for 40 hours before treatment with 2μg/ml Staurosporine for an additional 5 hours. Cultures were co-stained with either Anti-ACTIVE^® Caspase-3 pAb or Anti-Cytochrome c mAb. Cy^®3-conjugated goat anti-rabbit or -mouse IgG was used as a secondary labeling antibody and the cells were visualized by confocal microscopy. (3252)

Notes: The Anti-ACTIVE^® Caspase-3 polyclonal antibody was used to immunohistochemically stain Newcastle Disease virus (NVD) -infected chicken spleens. Sections were deparaffinized, peroxidase treated and microwaved for 10 minutes to retrieve antigens. The Anti-ACTIVE^® Caspase-3 polyclonal antibody was utilized and detected with a biotinylated anti-rabbit antibody, steptavidin-phosphatase and DAB. See the original article for more information on sample treatment for immunohistochemical analysis of this tissue. (2831)

Notes: Researchers used the Anti-ACTIVE^® Caspase-3 polyclonal antibody to immunocytochemically stain transiently transfected CHO-K1 (Chinese Hamster) cells overexpressing PLSCR1 or PLSCR2. Confocal microscopy, in conjuction with an immunofluorescent Alexa fluor 488 secondary antibody, was used to visualize samples. The authors provide details of the staining procedure. They used a 1:500 dilution of the Anti-ACTIVE^® Caspase-3 pAb and counterstained samples with propidium iodide. Representative microscopic images were displayed as well as a graphical depiction of the percent of active caspase-3-positive cells. (2832)

Notes: Anti-ACTIVE^® Caspase-3 antibody was used in immunocytochemical analysis of human neuroblastoma (SK-N-SH) cells transiently transfected with various vectors and an expression vector expressing huntingtin exon 1 (httEx1) containing 103 glutamines fused to enhanced green fluorescent protein (EGFP). The authors also used Promega’s SB 203580 MAP kinase homologue, p38α, p38β and p38β2 inhibitor in both COS-7 and SK-N-SH huntingtin exon 1-transfected cell cultures. Decreased nuclear fragmentation was reported when 1 or 10μM SB 203580 inhibitor was added to the transfected cell cultures. (2828)

Notes: The apoptotic nature of neuron cell death via a chemokine-activated cell–cell communication system involving microglia was characterized. Hippocampal pyramidal neurons were obtained from embryonic day 17 rat brain. Cells were exposed to gp120_IIIB and stained for neuronal death by apoptosis using the DeadEnd Colorimetric TUNEL System. Neuronal death was also detected by immunocytochemistry using the Anti-ACTIVE^® Caspase-3 pAb (1:250 dilution). (2352)

Notes: The authors used the Anti-ACTIVE^® Caspase-3 pAb to detect apoptotic cells in dissociated cell cultures created from embryonic mouse brain. (2600)