Notes: The SP6 and T7 RNA Polymerases were used to produce RNA probes for Northern Analysis. The authors examined the half-life of the m4 muscarinic receptor in PC12 cells and transformed PC12 cells following treatment with either murine 2.5S NGF, human recombinant basic FGF or human recombinant EGF. PC12 cells were also treated with the three factors, nuclei isolated, and nuclear run-on transcription assay were performed. (0814)

Notes: T7 RNA Polymerase and SP6 RNA Polymerase were used to produce RNA probes labeled with digoxygenin-11-UTP. Transcripts were made in the presence of RNasin^® Ribonuclease Inhibitor in a standard transcription reaction. The ribonucleotides ATP, CTP and GTP were used at 1mM. Both UTP and digoxygenin-11-UTP were used at 0.5mM in the reaction. The RNA probe was used for in situ hybridization. (1563)

Notes: The Dual-Luciferase^® Reporter Assay System was used to study transfections of IMR32 and NIH3T3 cells with firefly luciferase vector (pGL3 Basic) constructs. Transfections were controlled with co-transfected pRL-CMV Vector. Tth DNA Polymerase was used as a high temperature reverse transcriptase. SP6 RNA Polymerase was used to produce probes for RNase protection assays. (0558)