PureYield™ Plasmid Midiprep Start-Up Kit
Plasmid Midiprep System, Vacuum Manifold and Free Vacuum Pump
- Up to 200µg of plasmid from 50ml culture
- Transfection-quality plasmid DNA in 30 minutes
- No tedious, gravity-drip columns or post-elution alcohol precipitation
- For additional voltage options, contact Technical Service
Catalog Number:
Choose voltage required
Catalog Number: A6742
Special Offer: Switch to the Vacuum Format and get a Free Welch® Vacuum Pump! For a limited time PureYield™ Plasmid Midiprep Start-Up Kits are available. Purchase a PureYield™ Plasmid Midiprep System and a Vac-Man® Vacuum Manifold, and get a free Welch® Vacuum Pump! Each vacuum pump bundle is available in 3 formats. If you are not sure which format is right for your geographic area, contact your Promega representative or an authorized Promega distributor.
The PureYield™ Plasmid Midiprep System provides a rapid method for purification of 100–200μg of transfection-quality plasmid DNA from 50ml bacteria cultures. Using the vacuum protocol, plasmid isolation is complete in as little as 30 minutes, significantly reducing time spent on purification compared to silica resin or other membrane-column methods. An alternative protocol allows purification of over 400μg of high-copy-number plasmid from 250ml of E. coli culture.
The system incorporates a unique Endotoxin Removal Wash designed to remove substantial amounts of protein, RNA and endotoxin contaminants from purified plasmid DNA. Removal of contaminants improves performance in sensitive applications such as eukaryotic transfection, in vitro transcription and coupled in vitro transcription/translation. Purification is achieved without isopropanol precipitation of purified plasmid DNA or extensive centrifugation.
PureYield™ Plasmid Midipreps can be used with centrifugation or vacuum-based protocols.
Protocols
No protocols available
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
Eluator™ Vacuum Elution Device |
A1071 | 1 × 4 each | View Product |
|
PureYield™ Plasmid Midiprep System |
A2495 | 1 × 100 preps | |
|
Welch Vacuum Pump for Continental Europe Electrical |
A6722 | 1 × 1 each | |
|
Vac-Man® Laboratory Vacuum Manifold, 20-sample capacity |
A7231 | 1 × 1 each | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Resources
No related resources available
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Frequently Used With
FuGENE® 6 Transfection Reagent
Proven, dependable performance. Best for everyday use with common cell lines.
E2691, E2692, E2693
ViaFect™ Transfection Reagent
High-efficiency, low-toxicity transfection of many cell types, including iPS cells.
E4981, E4982
HB101 Competent Cells
E. coli strain ready for transformation. No blue/white selection.
L2015, L2011
BL21(DE3)pLysS Competent Cells
Ready-to-transform E. coli cells with IPTG-inducible protein expression from the T7 promoter.
L1195, L1191
