Wizard® Plus SV Minipreps Start-Up Kit
Plasmid Miniprep System, Vacuum Manifold and Free Vacuum Pump
- 1–20μg high-quality plasmid DNA from 1–10ml cultures
- Process up to 20 minipreps in 45 minutes
- Choice of spin or vacuum purification formats
- For additional voltage options, contact Technical Service
Catalog Number:
Choose voltage required
Catalog Number: A6762
The Wizard® Plus SV Minipreps DNA Purification System, a silica membrane-based system, provides a simple and reliable method for rapid isolation of plasmid DNA. The entire miniprep procedure can be completed in 45 minutes or less, depending on the number of samples processed. Using the system, plasmid DNA can be purified from 1–10ml of overnight E. coli culture. The purified plasmid DNA meets a target of >98% accuracy over 500 bases using pGEM®-3Zf(+) Vector and can be used directly for automated fluorescent BigDye® terminator DNA sequencing as well as for other standard molecular biology techniques without further manipulation. It also can be used for in vitro transcription reactions when supplemented with a ribonuclease inhibitor such as Recombinant RNasin® Ribonuclease Inhibitor (Cat.# N2511).
Wizard® Plus SV Minipreps DNA Purification System allows a choice of centrifugation (spin) or vacuum-based methods for plasmid purification. Vacuum Adapters allow the use of a vacuum manifold and vacuum source for DNA purification.
Protocols
No protocols available
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
Vacuum Adapters |
A1331 | 1 × 20 each | |
|
Wizard® Plus SV Minipreps DNA Purification System + Vacuum Adapters |
A1470 | 3 × 250 preps | |
|
Welch Vacuum Pump for Continental Europe Electrical |
A6722 | 1 × 1 each | |
|
Vac-Man® Laboratory Vacuum Manifold, 20-sample capacity |
A7231 | 1 × 1 each | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Resources
Other Resources
Related Products
Similar Products
Wizard® SV 96 and SV 9600 Plasmid DNA Purification Systems
96-well manual or high-throughput plasmid purification methods.
A2250, A2255, A2258
PureYieldâ„¢ Plasmid Miniprep System
Up to 15μg transfection-ready plasmid from 3ml cultures in 10 minutes.
A1223, A1222
PureYieldâ„¢ Plasmid Midiprep System
Up to 200µg of transfection-ready plasmid from 50ml cultures in 30 minutes.
A2492, A2495, A2496
Wizard® SV Gel and PCR Clean-Up System
Purifies DNA fragments from gel slices or PCR in 15 minutes.
A9281, A9282, A9285, A9283, A9284
Frequently Used With
pGEM®-T Vector Systems
T-vector for simplified cloning of PCR products.
A3600, A3610
FuGENE® 6 Transfection Reagent
Proven, dependable performance. Best for everyday use with common cell lines.
E2691, E2692, E2693
JM109 Competent Cells
E. coli strain ready for transformation; offers blue/white selection.
L2005, L1001, L2001