Identity Automation™ qPCR Setup Protocol for the PowerQuant® and Plexor® HY Systems on the Tecan Freedom EVO® Workstation
Instructions for Use of Product(s)
DC1000, DC1001, PQ5002, PQ5008
Literature # EP047
This document describes the automated protocol for the Identity Automation™ PowerQuant® and Plexor® HY Systems on the Tecan Freedom EVO® automated liquid-handling workstation. The automated method prepares 96-well PCR plates for DNA quantitation by real-time quantitative PCR (qPCR) of up to 86 unknown samples per plate when using a 4-point DNA standard curve or up to 80 unknown samples per plate when using a 7-point DNA standard curve.
Summary of Changes
The following changes were made to the 1/21 revision of this document:
1. Updated to include ability of method to process Unknowns and Controls supplied in 1.5ml or 2.0ml tubes.
2. Added new Sections 6, 11 and 12.
3. Updated disclaimers.
Revised 1/21.