Wizard® SV 9600 Plasmid DNA Purification System Technical Bulletin
Instructions for Use of Product(s)
A2258
Literature # TB292
This system requires the use of the Vac-Man® 96 Vacuum Manifold or compatible vacuum manifold. Plasmid DNA is purified from bacterial lysates using a 96-well vacuum filtration step to simultaneously clear the bacterial lysate and bind plasmid DNA, eliminating the need for centrifugation. Removal of the Lysate Clearing Plate and subsequent washing of the bound plasmid DNA requires no disassembly of the manifold, and filtrate waste products are delivered directly to a vacuum trap, eliminating the need to empty waste collection vessels during plasmid DNA recovery. DNA is collected by elution into a 96-well plate.
Summary of Changes
The following changes were made to the 12/25 revision of this document:
1. Document contents were moved to a new template that includes a new cover image and fonts.
2. The protocol in Section 3.B was updated to recommend removing cell medium by pipetting.
3. An expired patent statement was removed.
4. Third party trademarks were updated.
5. Removed Related Products section. See www.promega.com for related products.
6. Storage temperatures were updated to modern temperature ranges.
Revised 12/25.